Molecular Identification of Sarcoptes scabiei Isolate Local Rabbit on Several Areas in East Java of Indonesia
Nunuk Dyah Retno Lastuti, Anwar Ma-ruf, Lucia Tri Suwanti, Poedji Hastutiek Farah A-idah Nurreza

Universitas Airlangga

Abstract

ABSTRACT In Indonesia, genetic research for Sarcoptes scabiei in rabbits is still limited. The development of diagnostic kits and sub-unit vaccines for the prevention of scabies is urgently needed because several studies state that S. scabiei is likely to be resistant to anti-scabies drugs such as ivermectine. Early studies carried out genetic identification of S.scabiei mites from scabies infected rabbit from several rabbit farms in East Java. The purpose of this research was to identify the COX-1 gene from S. scabiei-s mitochondrial DNA, isolated rabbits from Pacet and Lawang, East Java, and used specific primers with target of 290 bp. The research methods were as follows: isolation and identification of S. scabiei from rabbits infected with scabies, DNA extraction with QIAamp DNA mini kit (Qiagen, Hilden, Germany), amplification by PCR. PCR products from S. scabiei isolates from Pacet and Lawang rabbits produced bands of around 290bp with 2% agarose gel electrophoresis. The research still needs to be continued for the analysis of sequencing of the COX-1 S. scabiei gene to identify the homology between rabbits from various regions with GenBank data.

Keywords: East Java, Genetic, Local Rabbits, Sarcoptes scabiei, Scabies

Topic: Health and Medicine

Link: https://ifory.id/abstract-plain/xebVvr2JHkgW

Web Format | Corresponding Author (Nunuk Dyah Retno Lastuti)