Decreasing lignin, cellulose and hemicellulose levels in fermentation of bagasse using Phanerochaete chrysosporium
Sri Rulianah, Prayitno, Nanik Hendriati and Ferina
Chemical Engineering Department, State Polytechnic of Malang, Malang, Indonesia
Abstract
Lignocellulose is an abundant organic component consists of three polymers namely cellulose, hemicellulose and lignin which it is the main sources of plant biomass. The lignin, cellulose and hemicellulose can degraded using Phanerochaete chrysosporium. The aim of this research was to determine the effect of fermentation time and bagasse addition to reduce the levels of lignin, cellulose and hemicellulose in fermentation process using Phanerochaete chrysosporium. This research was conducted by fermentation of bagasse which has been dried and reduced in size in the form of powder (30-60 mesh) using NLM (Nitrogen Limited Media). Changing variables in this study were fermentation time (17, 20, 23, 26, and 29 days), and concentration of bagasse addition (3,4 and 5%) from NLM. The product fermentation was filtered using a vacuum pump, the pulp was analyzed for levels of lignin, cellulose and hemicellulose by the Van Soest method. The results of the research showed that the longer fermentation time causing higher reduction levels of lignin, cellulose, and hemicellulose. In the best operating conditions, the fermentation time was 29 days, and the bagasse addition of 3% obtained the largest decrease in lignin by 93,273%, cellulose 68.0366%, and hemicellulose 54.495%.
Keywords: bagasse, cellulose, hemicellulose, lignin, Phanerochaete chrysosporium
Topic: Chemical Engineering