Effect of Differences in the Form of Photobioreactor Prototypes and Aeration Period on Chlorella sp Cell Growth in Co-Culture with Bacteria
Lirofiatillah (1), Sitoresmi Prabaningtyas (1.a), Murni Saptasari (1), Eko Agus Suyono (2)

(1) Department of Biology, Faculty of Mathematics and Natural Sciences,
State University of Malang, Jl. Semarang 5 Malang 65145, Indonesia
(2) Faculty of Biology, Gadjah Mada University, Bulaksumur, Caturtunggal, Kec. Depok, Kabupaten Sleman, Daerah Istimewa Yogyakarta 55281, Indonesia

Abstract

Microalgae are photosynthetic microorganisms that produce oil which have the potential for fuel production. Increasing the growth of microalgae can be done by co-culture. The purpose of this study was to determine the effect of photobioreactor prototype shape, aeration period and interaction between the aeration period and the photobioreactor prototype form on the growth of co-culture Chlorella sp cells with bacteria. This study uses a Completely Randomized Design (CRD). The form of the prototype used is the cube and horizontal plate. The treatment of different aeration periods consists of 6A: 18TA; 12A: 12TA; 18A: 6 TA and 24 A: 0 TA (A = Aeration, TA = No Aeration). The study was carried out by observing Chlorella sp cell density in co-culture with bacteria every 7 days. The results showed that the optimal prototype shape was a horizontal plate with an average cell density of 11.48x10 4 cells/ml. Horizontal plates are also often referred to as flat panels. This flat panel photobioreactor has a length and height and a narrow width to allow for greater penetration of light. Aeration serves to prevent the deposition of microalgae and the homogenization of the environment, so that all cells can obtain light and food, facilitate gas exchange between the cultivation and air environments. The optimal aeration period was 12 hours aeration: 12 hours not aerated with a mean cell density of 38.8 x10 4 cells/ml. The results showed that there was a significant difference in the treatment of prototype forms and aeration periods towards the growth of Chlorella sp cells co-culture with bacteria. The results also showed that there was no interaction between the different aeration periods and the photobioreactor prototype on the growth of co-culture Chlorella sp cells with bacteria.

Keywords: Prototype, photobioreactor, aeration, co-culture

Topic: Microbiology

Link: https://ifory.id/abstract-plain/zNZYgRw9BA76

Web Format | Corresponding Author (Lirofiatillah Lirofiatillah)