Strategies to Increase growth Early Embryo Stages of Bovine in achieving Blastocysts In Vitro
E Damayanti 1, H Sonjaya 2, H Hasbi 2, S Baco 2
1Postgraduate Student of Agricultural Study Program of Hasanuddin University Jl. Perintis Kemerdekaan KM 10 Makassar 90245, Indonesia.
2Departement of Animal Production, Faculty of Animal Husbandry, Hasanuddin University Jl. Perintis Kemerdekaan KM 10 Makassar 90245, Indonesia
Abstract
The growth of bovine embryos in vitro begins with fertilization is the unification of male and female gametes in the fertilization medium into zygote. After unification, the embryo will cleavage from two cells until they reach a blastocyst which can be transferred to the recipient. One of indicator the success of bovine embryo culture in vitro is the ability of the embryo from an early stage of development to reach blastocyst. To achieve this aim an embryo enhancement strategy is needed to reach the Blastocyst stage. Various strategies that can be carried out for the improvement include the use of an appropriate maturation medium, removed cumulus cells and the addition of hormones to the culture medium. The use of CR1aa medium in culture medium can reach 27.0% of embryos that reach blastosis of total fertilized oocytes. removal of cumulus cells after 48 hours of fertilization can increase the development of embryos reaching the blastocyst stage. In addition, by increasing the TGFβ hormone 1 ng / ml plus bFGF 50 ng / ml can increase the levels of 2 cells by 314 reaching the blastocyst level of 30 embryos.
Keywords: Bovine, Embryo, cleavage, Blastocyst
Topic: Animal reproduction