SEPARATION OF CALOPHYLLOLIDE FROM CALOPHYLLUM INOPHYLLUM OIL
Brilian Pamungkas, Claudia Shantika Primaswari, Setiyo Gunawan*
Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember (ITS), Surabaya 60111, Indonesia
*Corresponding author:
Email: gunawan[at]chem-eng.its.ac.id
Tel: +62-31-5946240; Fax: +62-31-5999282
Abstract
Nyamplung (Calophyllum inophyllum) are widely spread in Indonesia and they are known to have many advantages. It has various benefits that can be utilized from root, stem, leaf, until seed. C inophyllum-s seed contains bioactive compound called calophyllolide. However, the utilization of C. inphyllum-s seed is still limited because they contain harmful toxins. So, C. inophyllum-s seed are generally used and investigated as a raw material of biodiesel. The aim of this research was to find the best condition to isolate calophyllolide, to know the yield of isolated calophyllolide and its purity percentage from crude C inophyllum oil. By far, the current research on calophyllolide is to extract the substance form C. inophyllum-s shell nut without further treatment on the shell nut. In this work, calophyllolide was separated from crude oil by silica gel adsorption and batchwise solvent extraction by using a mixture of hexane as non-polar solvent and methanol-water (9:1) as polar solvent. Liquid Liquid Extraction (LLE) was performed to purify the calophyllolide from polar liquid fraction produced from batchwise solvent extraction by mixture of hexane and ethyl acetate with solvent-PLF ratio 5:2. Ethyl acetate concentration in hexane used were 0%, 5%, and 9%. Each fraction obtained was tested qualitatively using Thin Layer Chromatography (TLC) and quantitatively using Gas Chromatography (GC) to analyze calophyllolide mixture. The best separation method were obtained by batchwise solvent extraction and 5% ethyl acetate with 67,94% purity level.
Keywords: adsorption, batchwise calophyllolide , Calophyllum inophyllum, silica gel, solvent extraction.
Topic: Chemical Engineering