Influence of Erythrina crista-galli L. Extract as Natural Dye In Plant Histology Staining
Eko Susetyarini, Sri Wahyuni, Islakhul Kharoir, Husamah, Dwi Setyawan*
Department of Biology Education, University of Muhammadiyah Malang, Jl. Raya Tlogomas No. 246 Malang 65144, East Java, Indonesia
Abstract
The development of the use of preparations with natural coloring has been carried out a lot, but there is no one that shows optimal concentration. The purpose of this study was to determine the optimal concentration of Erythrina crista-galli L. flower extract as a natural dye based on the parameters of the quality and stability of the preparation. The type of research used is experimental research. This research method through several stages: extracting, diluting extracts with various concentrations (30%, 50%, 70%, 90%, and 100%), until making maceration preparations. Data collections were conducted twice, where the first and second take has an interval of one semester. Kruskall Wallis is used as a technique for analyzing data from research results. Both in the first and second observations, the concentration of 70% always produce the best quality preparations. Thus the Erythrina crista-galli L. flower extract has the potential as natural staining in histological preparations.
Keywords: Erythrina crista-galli L., natural staining, preparation histology
Topic: Botany