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The development of the use of preparations with natural coloring has been carried out a lot, but there is no one that shows optimal concentration. The purpose of this study was to determine the optimal concentration of Erythrina crista-galli L. flower extract as a natural dye based on the parameters of the quality and stability of the preparation. The type of research used is experimental research. This research method through several stages: extracting, diluting extracts with various concentrations (30%, 50%, 70%, 90%, and 100%), until making maceration preparations. Data collections were conducted twice, where the first and second take has an interval of one semester. Kruskall Wallis is used as a technique for analyzing data from research results. Both in the first and second observations, the concentration of 70% always produce the best quality preparations. Thus the Erythrina crista-galli L. flower extract has the potential as natural staining in histological preparations.