The Ability of Pineapple Callus Regeneration (Ananas comosus L.) from Sipahutar North Sumatra Indonesia With In Vitro Culture
Fauziyah Harahap (1*), Nikmatul K Harahap (1), Ely Djulia (1), Dirga Purnama (1), Herbert Sipahutar (1), Rosmayati (2), Suci Rahayu (2), Periseuein F Zega (1), Rifa Fadhilah Munifah Hasibuan(3)
1Department of Biology, Faculty of Mathematics and Natural Sciences, Medan State University, Jl. Williem Iskandar Pasar V Medan Estate, Medan, 20221, Indonesia
2 Department of Biology, Faculty of Mathematics and Natural Sciences, University of North Sumatera, Jalan Biotechnology No.1, USU Campus Medan, 20155, Indonesia
3Department of Agronomy and Horticulture, Faculty of Agriculture, Agriculture University, Bogor, Indonesia.
Abstract
Pineapple (Ananas comosus L.) from Sipahutar is an herbaceous plant from Sipahutar District. Pineapple canning industry sourced from Sipahutar pineapple has the opportunity to be developed in North Sumatra. Propagation through in vitro culture is an alternative method to solve this problem. The purpose of this research was to determine the regeneration ability of pineapple callus derived from callus induction with the addition of 2,4-D 1 ppm and BAP 1 ppm. The callus were regenerated using the addition of Kinetin (0; 0.5; 1; 1.5 ppm). Each treatment was carried out 4 times. The results showed: 1) the fastest time of emergence of shoots (5 days) came from 0.5 ppm, 2) formed the most shoots (8 shoots) resulting from the addition 0 ppm and 0.5 ppm, 3) the most number of leaves (7.75 leaves) resulted from light treatment with the addition kinetin 0.5 ppm and 1 ppm, 4) the longest leaf length (4.44 cm) from dark treatment with kinetin 0.5 ppm, 5) the highest plant height (5.32 cm) from dark treatment with 0.5 ppm kinetin. This shows that the concentration of 0.5 ppm kinetin with the dark treatment is the best concentration to stimulate the formation of pineapple shoots.
Keywords: Pineapple, kinetin, regeneration, in vitro
Topic: Biochemistry, Biotechnology and Pharmacy