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Pineapple (Ananas comosus L.) from Sipahutar is an herbaceous plant from Sipahutar District. Pineapple canning industry sourced from Sipahutar pineapple has the opportunity to be developed in North Sumatra. Propagation through in vitro culture is an alternative method to solve this problem. The purpose of this research was to determine the regeneration ability of pineapple callus derived from callus induction with the addition of 2,4-D 1 ppm and BAP 1 ppm. The callus were regenerated using the addition of Kinetin (0; 0.5; 1; 1.5 ppm). Each treatment was carried out 4 times. The results showed: 1) the fastest time of emergence of shoots (5 days) came from 0.5 ppm, 2) formed the most shoots (8 shoots) resulting from the addition 0 ppm and 0.5 ppm, 3) the most number of leaves (7.75 leaves) resulted from light treatment with the addition kinetin 0.5 ppm and 1 ppm, 4) the longest leaf length (4.44 cm) from dark treatment with kinetin 0.5 ppm, 5) the highest plant height (5.32 cm) from dark treatment with 0.5 ppm kinetin. This shows that the concentration of 0.5 ppm kinetin with the dark treatment is the best concentration to stimulate the formation of pineapple shoots.