TOTAL FLAVONOID LEVELS OF 70% ETHANOLIC EXTRACT OF (Sauropus androgynus (L.) Merr) LEAVES FROM THREE REGIONS WITH DIFFERENT ALTITUDE
Ni Putu Ermi Hikmawanti*, Hayati, Yeni Andriyani
Program Studi Farmasi, Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. Hamka, Jakarta
Abstract
Sauropus androgynus (L.) Merr or known as katuk is a family of Euphorbiaceae. This plant grows in the tropics. The aim of this study was to determine the total flavonoid levels of 70% ethanol extract of katuk leaves from three regions with different altitute. Extraction of katuk leaves was done by maceration method using 70% ethanol. Determination of flavonoid levels was carried out by the colorimetric method using AlCl3 reagent. Quercetin is used as a comparison. The absorbance measurements were carried out at the maximum wavelength of 434.50 nm using UV-Visible spectrophotometry. The results showed that the total flavonoid levels of 70% ethanol extract of katuk leaves from regions with low altitude (Bogor), medium altitude (Sleman) and high altitude (Bandung) are 8.56±0.63 mgQE/g, 4.67±0.30 mgQE/g and 9.72±0.24 mgQE/g, respectively. Thus, it can be concluded that there are differences on total flavonoid levels of 70% ethanol extract of katuk leaves from regions with different altitude.
Keywords: Total flavonoids, Katuk, Altitude, Sauropus androgynus (L.) Merr
Topic: Farmakognosi dan Fitokimia