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Sauropus androgynus (L.) Merr or known as katuk is a family of Euphorbiaceae. This plant grows in the tropics. The aim of this study was to determine the total flavonoid levels of 70% ethanol extract of katuk leaves from three regions with different altitute. Extraction of katuk leaves was done by maceration method using 70% ethanol. Determination of flavonoid levels was carried out by the colorimetric method using AlCl3 reagent. Quercetin is used as a comparison. The absorbance measurements were carried out at the maximum wavelength of 434.50 nm using UV-Visible spectrophotometry. The results showed that the total flavonoid levels of 70% ethanol extract of katuk leaves from regions with low altitude (Bogor), medium altitude (Sleman) and high altitude (Bandung) are 8.56±0.63 mgQE/g, 4.67±0.30 mgQE/g and 9.72±0.24 mgQE/g, respectively. Thus, it can be concluded that there are differences on total flavonoid levels of 70% ethanol extract of katuk leaves from regions with different altitude.