ANTIOXIDANT ACTIVITY FROM ETHANOL EXTRACT OF KUPA LEAVES (Syzygium polycephalum (Miq.) Merr & L. M. Perry) USING DPPH (2,2- diphenyl-1-picrylhydrazil) METHOD
Farid Perdana1*, Isye Martiani1, Dhanti M A1
1. Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Univesitas Garut Jl. Jati No. 42 B Tarogong Garut, Jawa Barat
*farid[at]uniga.ac.id
Abstract
Kupa plant is one of the endemic plants of Indonesia which is included in the tribe of guava (Myrtaceae). This study aims to determine the antioxidant activity of ethanol extract of kupa leaves (Syzygium polycephalum (Miq.) Merr & L. M. Perry), and to determine the class of metabolites available in simplicia and ethanol extract of kupa leaves. Extraction of kupa leaf simplicia was carried out by maceration method using ethanol 96%. The antioxidant activity test was carried out qualitatively and quantitatively. The qualitative antioxidant test was carried out by Thin Layer Chromatography using the developer chloroform: methanol: acidic format (8:2:1) and using a sitroborate and DPPH 0.2% spotting appearance. Quantitative testing of antioxidant ethanol extract of Kupa leaves was carried out using the DPPH method with vitamin C as a comparison. The TLC test results obtained a greenish color on the sitroborat and yellow on the DPPH with an rf value of 0.381. In the antioxidant test the kupa leaf ethanol extract obtained an IC50 value of 10,327 ppm which is a very strong antioxidant. And secondary metabolites found in simplicia and ethanol extract of kupa leaves are flavonoids, saponins, steroids / triterpenoids, quinones and phenols.
Keywords: Antioxidants, kupa leaves, ethanol extract, DPPH.
Topic: Biology