Endogenous Hormones at The Beginning of Leaf Growth after Vernalization of Garlic Bulb (Allium sativum L.) in Indonesian Local Varieties
Edi Siswadi1, Sekar Utami Putri1, Refa Firgiyanto1, Nuhak Nufitasari1, M. Zayin Sukri1, Cholyubi Yusuf2
1 Department of Agricultural Production, State Polytechnic of Jember, Mastrip Street 164 Jember, 68121, Indonesia
2 Department of Agribusiness Management, State Polytechnic of Jember, Mastrip Street 164 Jember, 68121, Indonesia
Abstract
Induction of garlic flowering can be done by vernalizing the seed tubers to trigger flowering. This study aims to determine the hormone status of IAA, GA3, at the time of initial growth of leaf tissue, after going through vernalization. Bulbs of Indonesian local varieties of Lumbu Kuning and Tawangmanu varieties were vernalized for 4 weeks, 8 weeks, and without vernalization treatment as a control. The contents of IAA and GA hormones in the leaf tissue of garlic plants aged 2 weeks were analyzed using method high performance liquid chromatography (HPLC). The results showed that the Lumbu Hijau variety with 4 weeks vernalization contained IAA of 1615,595 ng / g; GA3 is 7.297 ng / g. At 8 weeks vernalization the IAA content is 2169,475 ng / g; GA3 of 9,757 ng / g; In the Tawangmanu variety with 4 weeks vernalization containing IAA of 1459,168 ng / g; GA3 is 6.617 µg / g. Whereas for 8 weeks vernalization of IAA content of 2007,195 ng / g; GA3 is 8.315 ng / g.
Keywords: Garlic, Vernalization, IAA dan GA
Topic: Agriculture Engineering and Biotechnology