EFFECTIVENESS OF DAUN PEGAGAN (CENTELLA ASIATICA (L)URBAN) LEAF EXTRACT ON THE LEVEL OF IL-6 TO SPRAGUE DAWLEY RAT INDUCED STAPHYLOCOCCUS AUREUS
Ani1,Prihantono2,Risfah Yulianty3,Andi Nilawati Usman1, Sartini3, Irfan Idris4, Burhanuddin Bahar5.
1Department Of Midwifery, Graduated School, Hasanuddin University, Indonesia
2Department Of Onkology, Doctor Faculty, Hasanuddin University, Indonesia
3Department Of Pharmacy , Pharmacy, Hasanuddin University, Indonesia
4Physiology Departement, Doctor Faculty, Hasanuddin University, Indonesia
5Doctor Department, Doctor Faculty, Hasanuddin University, Indonesia
Abstract
Objective: This study aimed to analyze the effectiveness of centella asiatica leaf extract on IL-6 levels in the mammals of Sprague dawley mice induced by Staphylococcus aureus. Method : Type of Research True-Experiment is a laboratory experiment, with the design of the Post-test Only Control Design Group. The study was divided into four treatment groups consisting of five rats induced by Staphylococcus aureus 2 x 107 CFU / ml, 1 x 24 hours. Group I (negative control) is only given Na. CMC 0.5%, Group II (Positive Control) was given Cefadroxil antibiotics 45 mg/kgBB, group III was given centella asiatica leaf extract 100 mg/KgBB, group IV was given centella asiatica leaf extract combined with antibiotic Cefadroxil. Each group was given treatment twice a day (12 hours) for 5 days. Levels of IL-6 were measured on day 4 and day 7 after induction of Staphylococcus aureus, tested by ELISA technique and analyzed by One Way Anova test and Paired Samples T test. Results: The value of IL-6 levels in group III was 5.32 ± 0.55 pg/ml on day 4, and 3.49 ± 1.26 pg/ml on day 7. The results showed that there were differences in IL-6 levels in the group given the centella asiatica leaf extract (ρ = 0,017 < 0,05). Conclusion: Pegagan leaf extract can suppress pro-inflammatory cytokines after administration of treatment on mammals of Sprague Dawley mice induced by Staphylococcus aureus.
Keywords: Centella asiatica extract, Interleukin-6 (IL-6), Staphylococcus aureus, Sprague dawley.
Topic: Herbal medicine