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Objective: This study aimed to analyze the effectiveness of centella asiatica leaf extract on IL-6 levels in the mammals of Sprague dawley mice induced by Staphylococcus aureus. Method : Type of Research True-Experiment is a laboratory experiment, with the design of the Post-test Only Control Design Group. The study was divided into four treatment groups consisting of five rats induced by Staphylococcus aureus 2 x 107 CFU / ml, 1 x 24 hours. Group I (negative control) is only given Na. CMC 0.5%, Group II (Positive Control) was given Cefadroxil antibiotics 45 mg/kgBB, group III was given centella asiatica leaf extract 100 mg/KgBB, group IV was given centella asiatica leaf extract combined with antibiotic Cefadroxil. Each group was given treatment twice a day (12 hours) for 5 days. Levels of IL-6 were measured on day 4 and day 7 after induction of Staphylococcus aureus, tested by ELISA technique and analyzed by One Way Anova test and Paired Samples T test. Results: The value of IL-6 levels in group III was 5.32 ± 0.55 pg/ml on day 4, and 3.49 ± 1.26 pg/ml on day 7. The results showed that there were differences in IL-6 levels in the group given the centella asiatica leaf extract (ρ = 0,017 < 0,05). Conclusion: Pegagan leaf extract can suppress pro-inflammatory cytokines after administration of treatment on mammals of Sprague Dawley mice induced by Staphylococcus aureus.