Screening of Cellulase Producing Yeast Isolated from Indigenous Fermented Beverages of Indonesia
Dalia Sukmawati(a)(b), Gibran Sondana(b), Nabila Nov(b), Rifdah Hasanah(a), Hesham El Enshasy(c),(d), Sri Rahayu(b), Tri Handayani(b), and Rini Puspitaningrum(b)
(a) Biology Department, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
(b) Universitas Negeri Culture Collection, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
(c) Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia
(c) Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia
Abstract
The aim of this study is to demonstrate screening isolates of yeast with high cellulase activity from indigenous fermented beverages of Indonesia. This research conducted in Oktober 2018 until Maret 2019 in Microbiology Laboratory of Universitas Negeri Jakarta. The screening test of potential isolate to producing cellulase enzyme was perfomed on 53 yeast isolate from eight source indigenous fermented beverages that can grow in YMA medium with pH 2. Screening was carried out on CMC medium with difussion agar method. The cellulose activity of the organisms was measured diameter of a clear zone around the colony on Carboxymethyl Cellulose (CMC) agar media. The result of screening potential isolate to producing cellulase showed from 50 isolates, 17 isolate of yeast performed has potentially cellulose activity. They were selected according to their capabilities of degrading carboxymethyl cellulose (CMC). Among these 17 strains, 3 strains namely IL112, IL125 and IL78 showed higher levels of enzyme activity based on celulolytic index value.
Keywords: Yeast, Fermented, Cellulase
Topic: Biology