White tea (Camellia sinensis) supplementation in Sperm Extender on Ongole Bull Crossbreed Sperm Quality
Y. Laura1, W.T. Nugraha1, A. Adyatama1, Y. Indra1, L. Prawesti1, U. Fadlilah1, A. Istiarto2, D. Susanto2, Y.S. Sutanto2, Sudarto2

1 Animal Science Study Program, Faculty of Agriculture, Universitas Tidar, Magelang, Central Java, Indonesia
2 Technical Implementation Unit, Frozen Semen Hall, Special Region of Yogyakarta, Indonesia

Abstract

This study aims to understand how the antioxidant White tea extract can support sperm live in extender under 12 h at 5˚C storage. Sperm from productive three Ongole Bull Crossbreeds was collected at the morning twice a week. Fresh sperm were evaluated before dilution. Sperm extender used skim milk egg yolk as based extender and devided in to 3 groups, P0 (control), P1 (10% white tea extract), and P2 (20% white tea extract). The results for sperm motility and viability as compare with control (P<0,01). Motility of sperm P0, P1, and P2 were 85,00±5,00; 85,00±5,00; and 83,33±5,74 in 0h storage respectively then sperm motility of P0, P1, and P2 were 78,33±7,63; 76,67±5,77; and 0,00±0,00 respectively in 12h storage. Interaction between supplementation of white tea extract and time of storage were affected motility and viability of sperm (P<0,01). pH and abnormality did not affected by white tea extract addition but time of storage affect them (P<0,01). Adding antioxidant agents to sperm extender abundantly lead to oxidative stress through imbalance of Reactive Oxygen Species (ROS). It can be conclude that supplementation of white tea extract for 10% in to skim milk egg yolk sperm extender gave good results as compare as control.

Keywords: Antioxidant, Camelia sinensis, Sperm

Topic: Biology

Link: https://ifory.id/abstract-plain/Wr93QAgFVxtG

Web Format | Corresponding Author (Yosephine Laura Raynardia Esti Nugrahini)