Indonesia Conference Directory

This study aims to understand how the antioxidant White tea extract can support sperm live in extender under 12 h at 5˚C storage. Sperm from productive three Ongole Bull Crossbreeds was collected at the morning twice a week. Fresh sperm were evaluated before dilution. Sperm extender used skim milk egg yolk as based extender and devided in to 3 groups, P0 (control), P1 (10% white tea extract), and P2 (20% white tea extract). The results for sperm motility and viability as compare with control (P<0,01). Motility of sperm P0, P1, and P2 were 85,00±5,00; 85,00±5,00; and 83,33±5,74 in 0h storage respectively then sperm motility of P0, P1, and P2 were 78,33±7,63; 76,67±5,77; and 0,00±0,00 respectively in 12h storage. Interaction between supplementation of white tea extract and time of storage were affected motility and viability of sperm (P<0,01). pH and abnormality did not affected by white tea extract addition but time of storage affect them (P<0,01). Adding antioxidant agents to sperm extender abundantly lead to oxidative stress through imbalance of Reactive Oxygen Species (ROS). It can be conclude that supplementation of white tea extract for 10% in to skim milk egg yolk sperm extender gave good results as compare as control.