Maximum Inhibition Test of Siwak Extract (Salvadora Persica) Against Aggregatibacter actinomycetemcomitans
Arni Irawaty Djais, Hasanuddin Thahir, Muchammad Hatta, Harun Achmad, Vidya Toppe, and Melyanti Sari
Periodontology Department, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Microbiology Department, Molecular Biology and Immunology Laboratorium, Faculty of Medicine,
Hasanuddin University, Makassar, Indonesia
Pediatric Dentistry Department, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Abstract
Abstract Objective: To determine maximum inhibition of siwak extract against Aggregatibacter actinomycetemcomitans. Method: This study was an experimental laboratory with post-test only control group design. The study sample was Aggregatibacter actinomycetemcomitans in MHA medium. Total 24 samples were divided into 6 treatment groups, 5 groups of siwak-s extract with different concentration, 3.125%, 6.25%, 12.5%, 25%, 50% and 1 treatment group with DMSO control 5%. The inhibitory zone was measured after 48 hours incubation at 37 ° using calipers. Data analysis was performed with the Kruskal Wallis test. Results: The average diameter of inhibition zones at concentrations 3.125%, 6.25%, 12.5%, 25%, and 50% were 6.4 mm, 7.0 mm, 7.2 mm, 7.9 mm, and 8.6 mm, respectively. Conclusion: The maximum inhibition of siwak extract that can inhibit the growth of Aggregatibacter actinomycetemcomitans bacteria as one of the pathogenic bacteria that causes periodontal disease was siwak extract with a concentration of 50%
Keywords: Aggregatibacter actinomycetemcomitans; Maximum inhibitory test; Siwak-s extract
Topic: Herbal medicine