THE ROLE OF PULSED ELECTRIC FIELD (PEF) WITH THERMOCONTROL MODIFICATION AS AN ELICITOR TO INCREASE BIOACTIVE COMPOUND IN LOCAL SOYBEAN
Jaya Mahar Maligan*, Megy Wida Fitria, Widya Dwi Rukmi Putri
Department Agricultural Product Technology, Agricultural Technology Faculty, Brawijaya University
Abstract
Soybean is a agricultural commodity, being third in harvest amount after paddy and corn. The processing of soybean using heat causes the bioactive compounds to decrease. There is another way to increase the bioactive compounds in soybeans. Elicitation is a method that functions to increase biosynthesis of secondary metabolite formation in plants by using elicitor. This research used factorial Randomized Block Design method with 2 factors which were temperature (25oC, 35oC and 45oC) and pulsed field (3 kV, 5 kV and 7 kV). Electrical voltage test result from device obtained input voltage 0,76 V resulted 3 kV, 1,04 V resulted 5 kV, 1,4 V resulted 7 kV. Research results on soybean extract obtained total phenol as much as 2,76 mg GAE/g, total flavonoid 0,31 mg QE/g, tannin content 0,18 mg/g, IC50 as much as 626,95 ppm. Whereas for the results of extraction of soybeans in the best treatment was obtained at the pulsed field of 3 kV and temperature of 25oC. Total phenol result was 2,92 mg GAE/g, total flavonoid 0,37 mg QE/g, tannin content 0,19 mg/g, antioxidant activitiy IC50 128,84 mg/L
Keywords: Soy Bean, Elicitation, Pulse Electric Field, Temperature
Topic: AGRICULTURAL PRODUCT TECHNOLOGY