THE CHANGE OF STOMATA AND CROMOSOMES IN Celosia cristata PUTATIVE MUTAN BY GAMMA IRRADIATION
Syarifah Iis Aisyah1, Dhieni Hayati1, Krisantini3, Waras Nurcholis2,3*
1Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor
2Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor
3Pusat Studi Biofarmaka Tropika, Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor
Abstract
Celosia cristata is a crucial medicinal and ornamental plants. The gamma irradiation with in vitro mutagenesis can be used to form economically developed mutants. The effects of gamma-ray irradiation on stomata and chromosome of in vitro leaves cultures of C. cristata were evaluated. Leave cultures were gamma-irradiated at 0-75 Gy, and the population at third-generation (MV3) was used to assess the stomata and chromosome in C. cristata. The result demonstrated that the treatments of gamma-ray irradiation could be useful to change anatomy of stomata and chromosome of C. cristata putative mutants. Stomata density was found higher in dose 75 Gy with value 101.4 /mm2. The chromosomes of putative Celosia mutants were anatomically changed, which compared to control plantlets. This result indicated that the gamma-irradiated treatment changed DNA in the nucleus of C. cristata plantlets.
Keywords: Celosia cristata, chromosome, stomata, in vitro
Topic: Bioteknologi dan Molekular Tanaman Obat