Indonesia Conference Directory

Celosia cristata is a crucial medicinal and ornamental plants. The gamma irradiation with in vitro mutagenesis can be used to form economically developed mutants. The effects of gamma-ray irradiation on stomata and chromosome of in vitro leaves cultures of C. cristata were evaluated. Leave cultures were gamma-irradiated at 0-75 Gy, and the population at third-generation (MV3) was used to assess the stomata and chromosome in C. cristata. The result demonstrated that the treatments of gamma-ray irradiation could be useful to change anatomy of stomata and chromosome of C. cristata putative mutants. Stomata density was found higher in dose 75 Gy with value 101.4 /mm2. The chromosomes of putative Celosia mutants were anatomically changed, which compared to control plantlets. This result indicated that the gamma-irradiated treatment changed DNA in the nucleus of C. cristata plantlets.