Stimulatory Effect of the Methanolic Extract, n-Hexane Insoluble and Soluble Fraction of Parijoto Fruit (Medinilla speciosa Blume) on the Quantity Spermatozoa of Male Sprague Dawley Rats
Rina Wijayanti (a*), Subagus Wahyuono (b), Ika Puspita Sari (c), Dicky Moch Rizal (d)
a) Departement of Pharmacy, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia
*wijayanti[at]unissula.ac.id
b) Departement of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
c) Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
d) Departement of Physiology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
Abstract
One of the health problems is infertility, with an incidence of 15-20% of around 50 million couples, which men account for about 50% of all cases. Parijoto fruit (Medinilla speciosa Blume) is empirically used to in-crease fertility. The purpose of this study was to determine the stimulatory effect of methanol extract, insolu-ble and soluble fraction of n-hexane parijoto on the quantity spermatozoa. This study used 28 male SD rats, divided into VI groups, where group I was normal; group II; III; IV; are methanol extract groups with doses 100 mg/kgBW; 250 mg/kgBW; and 500 mg/kgBW, group V is n-hexane insoluble fraction 500 mg/kgBW, and group VI is n-hexane soluble fraction 500 mg/kgBW, each given orally for 14 days. The parameter observed was the quantity spermatozoa. The data obtained were analyzed using Kruskal-Wallis, and Mann-Whitney. The results showed the mean quantity spermatozoa in groups I, II, III, IV, V, and VI were respectively 26.8±1.6; 27.1±1.3; 30.4±1.3; 43.5±1.3; 67.1±2.8; and 50.1±1.9 (million/ml ejaculate). There were significant differences between groups I and groups IV, V and VI. Methanol extract, insoluble and soluble fraction of n-hexane parijoto fruit were able to provide a stimulating effect on the quantity spermatozoa in SD male rats.
Keywords: Methanol extract of Parijoto (Medinilla speciosa Blume); N-hexane insoluble and soluble fraction of Parijoto (Medinilla speciosa Blume); Quantity spermatozoa.
Topic: Basic and Clinical Medical Sciences