Supplementation of nucleotides to enhance performance and immune responses of Asian Seabass (Lates calcarifer)
Sri D. Hastuti1,2*, James L. Munro1, Stephen B. Pyecroft1, Mary D. Barton3, Maurizio Costabilec3,4, Brett Glencross5
1School of Animal and Veterinary Sciences, The University of Adelaide, Australia
2Department of Aquaculture, University of Muhammadiyah Malang, Indonesia
3School of Pharmacy and Medical Sciences, University of South Australia, Australia
4Centre for Cancer Biology, University of South Australia and SA Pathology, Frome Road, Adelaide, SA 5000, Australia
5Institute of Aquaculture, University of Stirling, United Kingdom
*Corresponding author: sri.hastuti[at]adelaide.edu.au
Abstract
Abstract Growth performance and immune responses of Asian seabass (Lates calcarifer) was assessed after being fed diet containing different levels of a commercial nucleotide (Optimun®) and a purified mixture of nucleotides containing AMP (Adenosine monophosphate), IMP (Inosine monophosphate), UMP (Uridine monophosphate), GMP (Guanidine monophosphate) and CMP (Cytidine monophosphate) at equal amounts. Six nucleotide supplemented diets and a control diet were used in this study namely O1 (Optimun 0.25%), O2 (Optimun 0.5%), O3 (Optimun 0.75%), P1 (0.25% nucleotides), P2 (0.5% nucleotides), P3 (0.75% nucleotide) and C (control/no supplemented nucleotides). Juvenile Asian seabass with an average weight of 13.19 ± 0.58 g (mean ± SD) were fed the treatment diet at 3% body weight per day for six weeks. The study revealed that the different inclusion level of nucleotide did not significantly affect the growth and immune responses of Asian seabass. However, diet P2 with supplementation of 0.5% nucleotide gave higher growth performance, serum total protein, albumin and globulin compare to other diet groups.
Keywords: Asian seabass, Growth performance, Immune responses, Nucleotides diet
Topic: Environmentally Sustainable Agriculture