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This research aimed to identify the profile of Higher Order Thinking Skill (HOTs) instrument in the questions of students- learning outcome assessment in chemistry for Senior High School in Malang City. This study used descriptive qualitative with a content analysis method. The data were collected by documentation. The populations were all Senior High Schools in Malang City. The samples were determined using cluster random sampling technique and were represented by SMAN 3, SMAN 8, and SMAN 9 Malang. The data used in this study were 576 questions of learning outcome assessment in chemistry used in those three schools, including 201 question for class X and 366 question for class XI, respectively. The finding validity was tested based on the analysis results of rater 1 and 2 using interrater and Cohen-Kappa reliability. The research results showed that the HOTs questions had been implemented, and the percentages of HOTs questions used in class X and XI were 9.00% and 13.39%, respectively. The validity test indicated a strong-perfect interpretation with the agreement coefficient value of 0.61-0.86. The research results are expected to be an evaluation in improving the quality of education, especially in the chemistry for Senior High School in Malang City.