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In recent times, there is growing interest to develop advanced materials for food packaging applications which can improve food safety and shelf-life. Active food packaging systems has been used as an effective method to enhanced the food safety and shelf-life. Chitosan nanoparticles represents interesting properties which makes it a suitable material for active packaging system. The stability and properties of chitosan nanoparticles can be enhanced by the modification of chitosan using biopolymer and bioactive compound. In this study, the modified chitosan nanoparticles were prepared via emulsion-based self-assembly technique using Poly Vinyl Alcohol (PVA) and acrylate polymer. Bioactive compound was encapsulated in chitosan nanoparticles to enhanced the property of active packaging materials. The systematic condition in preparation was studied. The characterization and morphology of the nanoparticles were investigated by Dynamic light scattering (DLS) and Transmission electron microscopy (TEM) analysis. The obtained results indicate that the modified chitosan nanoparticles may be a promising material for active food packaging materials.