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Polyethylene glycol (PEG) coated magnetite Fe3O4 nanoparticles has been successfully shyntesized by using chemical co-precipitation method with variation concentrations. The magnetite Fe3O4 nanoparticles used as a bimolecular labels (nano-tags) exhibiting a soft magnetic behavior with Ms of 77.16 emu/g and Hc of 49 Oe respectively and polyethylene glycol (PEG) as a stabilizer and dispersant. XRD patterns and transmission electron microscopy (TEM) images showed that Fe3O4 was well crystallized and it grew in their inverse spinel structure with an average size of around 10 nm. Fourier transform infrared spectroscopy (FT-IR) results suggest that PEG indicated with Fe3O4 via its carbonyl groups. Result of Vibrating Sample Magnetometer (VSM) shows that Fe3O4 have exhibited good magnetic response and easily attracted to a magnet placed beside with superparamagnetic behavior. Such Fe3O4 nanoparticles with favorable size and tunable magnetic properties are promising biosensor applications.