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Gold mining is the main source of waste tailings production that can pollute the environment. Gold phytomining is the process of extracting gold metal from the soil by a gold hyperaccumulator plant. In this study sunflower (Helianthus annuus) is used as the main agent of gold phytomining symbiosis with Arbuscular Mycorrhiza Fungi (AMF), Glomus sp. This study has two purposes. First, measuring the concentration of Au metal in sunflowers (Helianthus annuus) use symbiosis Glomus sp. Mycorrhiza, when accumulate gold metal. Second, determining the resistance of the stem height and number of sunflower (Helianthus annuus) leaves. The method used is through AAS and periodic measurements. The results obtain sunflower (Helianthus annuus) is able to accumulate gold. The highest concentration of Au metal is 137.81 mg/kg obtained at the treatment of 100 grams of mycorrhiza, while the lowest concentration of Au metal is 35.24 mg/kg obtained at the treatment of 0 gram mycorrhiza. The 40 grams of mycorrhiza has the most significant effect related to sunflower resistance (Helianthus annuus) when absorbing gold metal because it produces plants with a stem height of 13 cm and the highest number of leaves is 10 leaves.