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The purpose of this study is to develop a learning module based on laboratory research on Kebiul seed extract test to increase platelet count in mice. Twenty-five mice were grouped into five, group K- , mice were given sesame oil orally, K +, mice were given aspirin, and group P1, P2 and P3, mice were given Kebiul seed extract at a dose of 0.028 g / kgbw, 0.056 g / kgbw and 0.084g / kgbw. This laboratory research is then packaged as a supplementary module that was developed using the 4D model. The quality of the module can be identified by validation of expert judgment. The ability to think critically is measured using indicators focusing questions, considering whether the source is reliable or not, the indicators conclude by induction thinking and considering the results of induction, and identifying assumptions. Kebiul seeds with a dose of 0.056 g / kg bw are the most effective way to increase platelet counts in mice and have the potential to be used as a medicine to increase platelet count in DHF patients. Based on the results of expert validation, media experts gave a value of 91.9%, material experts amounted to 86.8% and module experts amounted to 86.4%, the modules were categorized as very feasible to use, student responses to the modules amounted to 85.80% (very good and effective) and feasible to use.