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Laundry services develop as one of the leading informal economic sector in Bali. Workload on laundry worker is due to monotonous process. Workloads that are not in accordance with the ability of workers could lead to excessive energy use, resulting in fatigue. The aim of this study was to find out the correlation between workload and fatigue rate among the laundry workers in Dalung Village, Kuta Utara District, Badung Regency. The method used in this study is analytical descriptive with cross-sectional approach. The subject in this study were laundry workers in Dalung Village, Kuta Utara District, Badung Regency. The amount of the sample was 40 people selected by purposive sampling method. Workload data was measured by calculating the working heart rate using pulsemeter. Fatigue data was measured by 30-item fatigue questionnaire with Likert scale. The relationship between workload and fatigue rate were analyzed by SPSS using Somers d test. The results were shown as follows the average of the working heart rate and fatigue rate : 91.6 ± 8.27bpm and 57.03 ± 9.68, respectively. The highest percentage of workload category was light workload (82,5%) and the highest precentage of fatigue category was moderate category (80%). There was positive significant relationship between workload and laundry worker fatigue rate ((p <0.05 and r=0.47). The conclusion could be drawn that there was a positive significant correlation between workload and laundry worker fatigue rate. It was suggested that the workers should be drink water and rest enough to reduce fatigue.