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This study aims to develop VirLab in the simulation of water content analysis and ash content analysis in food analysis course. Limited practical resources in tools and practical places encourage researchers to develop simulation media as a tool of learning. The ideal ratio demands between the tools of practice available and the number of students is a prerequisite for the prevalence of student learning opportunities in the practice room. The research method used is design based research. Stages of research consist of design, development, and testing. VirLab developed is a simulation that can be accessed online on the website. The thermogravimetric method is presented on VirLab for water content analysis simulation, and the dry fogging method is presented on VirLab for ash content analysis simulation. Expert judgment and users assess the Virlab to provide responses of visual communication aspects, material relevance aspects, and its benefit aspects as learning media. The results show that VirLab of water and ash content analysis simulation can be used as learning media for food analysis course.