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This study aims to determine the effect of interval running training on increasing VO2 Max on MAN Selat Tengah futsal Kapuas Regency. The research method used is the pre-experiment design method. The population in this study was the futsal player MAN Selat Tengah Kapuas Regency, who participated in futsal extracurricular totaling 30 people, while the sample of the study amounted to 20 people. The sampling technique uses purposive sampling. The results showed that the pretest variable increased VO2 Max in futsal players MAN Selat Tengah Kapuas Regency obtained an average of 13.1250 ml / kg / min with a standard deviation = 5.86550. Whereas the posttest variable increased VO2 Max on MAN futsal players in Selat Tengah Kapuas District obtained an average of 44.6100 with a standard deviation = 5.49937. T Test results obtained obtained t count = - 8,739> t table (23; 0,025) = 2,093, or with Sig. (2-tailed) = 0,000; it turns out that Sig. (2-tailed) <0.05 thus the tcount is significant. The conclusion is that there is an effect of interval running training on the increase in VO2 Max on MAN Selat Tengah futsal Kapuas Regency.