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Amylase is a starch hydrolyzing enzyme. This protein is very important for various industrial processes. Microorganisms are considered as the best source of amylase production. This study aims to isolate amylolytic bacteria from rice soil samples that have the potential to produce amylase enzymes. Paddy soil samples were collected from two locations, Percut Sei Tuan and Lubuk Pakam rice field. The isolates obtained were then characterized macroscopically and microscopically. Macroscopic characterization was carried out by observing the morphology of bacteria with a microscope while microscopic characterization was carried out by the Catalase Test, Endospora Staining Test and Gram Staining. From the isolation that has been done, 14 isolates have the potential to produce amylase enzymes. The obtained isolates were tested with Iodine staining. Isolates that had been macroscopically characterized showed results such as: irregular shape, slippery edges, branched, wavy, circular, having macroscopic characterization. raised and convex colony elevation, white and beige. The isolates which were characterized by the catalase test showed positive results for all isolates. Characterization with Endospora Staining showed 7 isolates positive while 7 isolates negative. For the characterization by gram staining, obtained 7 isolates were gram positive and 7 isolates were gram negative. Thus, from the two sample locations obtained amylolytic bacteria that potentially produce amylase enzymes.