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Rice (oryza sativa) bran is a byproduct generated in the process of rice milling. Moreover, subcritical method as a promising alternative solvent for chemical reaction, separation and extraction process and its nontoxic, inexpensive and invorementally benign, to increase levulinic acid in subcritical needs to be studied with ethanol-water mixture. While, ethanol is capable of hydrogen bonding and dipole-dipole interaction with organic acid and it is conveniently available and stable for hydrogenation. At the same time, carbon dioxide was use as a pressurizing gas and the solvent etanol-water mixture with the concentration (0:100, 25:75, 50:50) with the temperature was used at 160, 180 and 200 0C for 20, 30, 40, 50 and 60 minute reaction in the reactor.