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Death caused by Acute Coronary Syndrome (ACS) remains high because people do not have sufficient knowledge in interpreting the perceived sign and symptoms. This has an impact on the lack of people-s attitudes and beliefs in seeking health services. Providing information is a key factor to increase knowledge, attitude and belief toward ACS. This study aimed to determine the effect of health education on knowledge, attitudes and beliefs about acute coronary syndrome in Public Health Centre Tabanan III. This research implemented pre-experimental design. There 38 samples involved in this research which were selected through purposive sampling in the Public Health Centre Tabanan III. The health education was provided through 30 minutes presentation with the media of brochure and flyer. The data were collected by using ACS Response Index questionnaire and analyzed by using Wilcoxon Signed Rank Test. The result of Wilcoxon Signed Rank Test showed there was a statistically significant increase in knowledge, attitudes and beliefs after they were given health education. On the domain of knowledge, z = –5.40, p<0.001, r = 0.61, median score increased from pre-program (Md = 10) to post-program (Md = 16). On the domain of attitudes, z = –5.38, p<0.001, r = 0.61, median score increased from pre-program (Md = 8.5) to post-program (Md = 17). On the domain of beliefs, z = –5.40, p<0.001, r = 0.62, median score increased from pre-program (Md = 18) to post-program (Md = 21). There is an effect of health education on knowledge, attitudes and beliefs about acute coronary syndrome in Public Health Centre Tabanan III.