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Chlorpyrifos is an organophosphate insecticide which has molecular formula C9H11Cl3NO3PS and toxic. Bioremediation is one of environmental friendly method to remove chlorpyrifos as a contaminant because of the microbial enzymatic activity. The aim of this study is to remove chlorpyrifos with temperature and concentration of Aspergillus fumigatus variations. Aspergillus fumigatus fungi acts as bioremedian with chlorpyrifos degradation activity in the temperature range 25-35oC and concentration of A. fumigatus 0.5-1.5%. By using Gas-Chromatography Mass Spectrometer (GC-MS), the optimum chlorpyrifos degradation was achieved at 25oC with 1% concentration of A. fumigatus for 5 days in potato dextrose broth (PDB) liquid media. It shows that A. fumigatus has the ability to remove chlorpyrifos with the efficiency of chlorpyrifos removal reached 85.7% from the initial concentration of chlorpyrifos at 100 ppm.