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The development of learning media is inseparable from the rapid development of technology. The purpose of this study was to develop a learning media for biology practicum guides in the form of smartphone applications. This research was conducted using the ADDIE model (Analysis, design, development, implementation, and evaluation). The media feasibility test obtained an assessment of 90.28%, material experts 87.75%, biology teachers 91.37% and 88.46% students with very good interpretations. The increase in psychomotor ability test obtained a gain value of 0.49, which means there is an increase in psychomotor abilities of students with a moderate category. The conclusion obtained is that the application of the biology practicum guide has been successfully developed and is suitable for use in learning.