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Introduction: Osteoarthritis (OA) is a chronic degenerative disease associated with inflammatory process and free radical formation. It causes progressive joint cartilage damage. The rhizome of red ginger and cogon grass (RGCG) have anti-inflammatory and antioxidant properties. This present study aimed to evaluate the effects of RGCG treatment on nitrate level of plasmatic and synovial membrane tissue of Wistar rats. Materials and methods: Twenty five male Wistar rats were grouped into five; control (CTL), osteoarthritic rats (OA), and 3 different doses of treatment groups (P1, P2, P3). Each rat of OA and treatment groups were given 0,1 ml right knee intraarticular complete Freund-s adjuvant (CFA) injection to induce local osteoarthritic state. All treatment groups were orally administered with 40 mg/day of I. cylindrica decoction. However, P1, P2 and P3 groups were given 9, 18 and 36 mg of Z. officinale decoction each day for 10 days, respectively. Nitrate level was measured with nitric oxide colorimetric assay kit. Plasmatic nitrate data was analyzed by Kruskal-Wallis and continued with Mann-Whitney. However, nitrate level of synovial membrane tissue was using oneway ANOVA continued with Post Hoc. Result were considered to be significant at P<0,05. Results: Plasmatic nitrate level of treatment groups was 4.3 ± 0.55, 3.91 ± 2.39, and 3.64 ± 0.7 nmol/µL respectively in P1, P2, and P3. These were significantly lower than OA group (7.15 ± 2.68 nmol/µL, P<0,05). In addition, synovial membrane tissue nitrate level of treatment groups was 2.99 ± 1.18, 2.79 ± 1.09 and 2.87 ± 0.41 nmol/µL respectively in P1, P2, and P3. These were significantly lower than OA group (3.84 ± 1.56 nmol/µL, P<0,05). Conclusions: The administration of RGCG decoction reduced the level of nitrate both in blood plasma and synovial membrane tissue in osteoarthritic-induced Wistar rats. The combination of Zingiber officinale and Imperata cylindrica may have potential as an anti-osteoarthritis agent.