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Humans in the development of the times become assets for the organization. The important role of human resources for organizations is a decisive element in the activities of a company. Nurses are one of the important resources owned by hospital organizations. Nursing services are an integral part of health services in hospitals. To develop nurses to be more advanced and developing in order to achieve company goals, then one that is needed is satisfaction in work. Role conflict can affect satisfaction in the workplace. Stress can mediate these factors on job satisfaction. The formulation of this study is how job satisfaction is influenced by the determinants and stressors of hospital nurses. The purpose of this study was to analyze and assess the determinants of job satisfaction for nurses in hospitals and analyze how stressors had an impact on increasing job satisfaction for nurses in hospitals. The sample of this study was 170 respondents. The questionnaire returned as many as 160 respondents. This research method with quantitative design uses path analys. Role conflict has a negative influence on job satisfaction. Role conflict has a positive impact on work stress. Job stress has a negative impact on job satisfaction. Role conflict has a negative impact on job satisfaction mediated by work stress.