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Lake sludge and natural phosphate can be applied to the cultivation of various plants, but have not been applied to spinach cultivation. The aim of the study was to determine the effect of Lake Sludge dosage and Natural Phosphate dosage on growth and yield of unripe spinach. The experimental method with factorial RCBD used on this research. The results showed that the interaction between treatment of Lake Sludge (D) and Natural Phosphate (P) dosages had no significant effect on all observed variables. The Lake sludge dose of 150 g per polybag gives total oven dry weight per plant of 8.07 g or 61.1% higher compared to a dose of 0 g per polybag (D0). Natural Phosphate treatment has a very significant effect on the total oven dry weight variables per plant. Natural Phosphate doses of 40 g per polybag provide total oven dry weight per plant of 7.31 g or 39.5% higher than a dose of without Natural Phosphate (P0). Both of the regression analysis between the dosages of Lake Sludge and Natural Phosphate with total oven dry weight per plant there is a linear relationship.