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Indonesia most popular commodities are strawberries which have high economic value. The indicator quality of strawberries can be seen from level of maturity. Auxin hormones have function to encode fruit ripening genes such as FaPYR1 and FaCHS. This aim of this study to detect FaPYR1 and FaCHS genes in strawberry plants induced by indole-3-acetic acid (IAA). The method used planting strawberries (Fragaria x ananassa "Crystal"), IAA induction by three different concentrations (10 ppm, 30 ppm and 60 ppm), genomic DNA isolation, quantitative testing, FaPYR1 and FaCHS genes amplification with PCR and morphological data analysis using SPSS. The results of the analysis using SPPS showed that highest yield of leaf length, leaf width, stem height and stem diameter at concentration of 30 ppm. The highest number of leaves was found at concentration of 60 ppm.The lowest morphological results were found in the control. The highest concentration genomic DNA was found in the red stage fruit with a treatment of 30 ppm and the lowest concentration was found at the white stage at 60 ppm. The results of amplification using PCR showed positive results for the FaCHS gene in all treatments and negative results on the FaPYR1 gene in several treatments.