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Iron oxide material is widely available in nature and can be explored in nanomaterial research and can be developed to be applied in the fields of industry, health, and technology. Iron oxides that are available in nature can be classified into several types, one of which is hematite. Hematite material doped with Zn was successfully synthesized using coprecipitation. Mass variations (0.1 and 0.3g) of Zn doping have also been carried out to determine the hematite behavior towards the characteristics of ZnxFe2-xO3 nanoparticles. ZnxFe2-xO3 nanoparticles were successfully characterized by using X-ray Diffraction (XRD) to determine the effect of Zn doping on crystal structure and particle size of ZnxFe2-xO3 nanoparticle, magneto-thermal to determine the thermal behavior of ZnxFe2-xO3 nanoparticles. The grain size of ZnxFe2-xO3 nanoparticles with doping masses of Zn 0.1 g and 0.3 g were obtained for 5 and 60 nm, respectively. The magneto-thermal analysis shows that the SAR value of ZnxFe2-xO3 nanoparticles with a doping mass of Zn 0.1 g and 0.3 g respectively 1.017 and 1.075 W / g. While the antimicrobial analysis of ZnxFe2-xO3 nanoparticles showed that the zone of inhibition was strengthened by the addition of Zn doping mass through the S. Aureus bacterium. Thus, ZnxFe2-xO3 nanoparticles are very potential to become antimicrobial agents.