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Particulate matter (PM) plays a crucial role in altering the air quality, human health and materials. PM do not only affects humans health but damages the museums artefacts in many ways such as paint and materials deterioration, metal corrosion, fading of dyes and soiling of stone rubber, and textile. The main aim of this research is to quantify the mass concentration of suspended particulate matter in terms of respirable and inhalable particles at outdoors, indoors, and exhibition boxes of the National Museum Malaysia. This research was conducted for 32 days (16 weekdays and 16 weekends). The main target are the exhibition gallery A and B, and also exhibition boxes for those containing inorganic materials such as metals, stones, and ceramics. In total, 13 samples stations were surveyed, this includes three exhibition box each from Gallery A and B, indoor area of both Gallery A and B, lobby and four outdoor locations at the front entrance, rear entrance, right and left-wing of the building. Cyclone sampler head and Casella 7-Holes were used to capture respirable and inhalable samples respectively. Result revealed that most of the average mass concentration obtained in all sample stations were beyond limited guidelines stated by the Department of Safety and Health (DOSH) and Department of Environment (DOE). The average mass concentrations of inhalable dust sampled at the metal and ceramic exhibition box of Gallery A at weekdays was the highest that is 0.4688 mg/m3, which is about 213 times more than the required standards. However, lower values were obtained for the respirable particles, but they were found to still beyond standard DOE guidelines for PM10 that is 0.004 mg/m3. The highest respirable particles were found at the ceramic exhibition box with 0.3788 mg/m3 for the average mass concentration. Further analysis was made by comparing the ratio of respirable particles to inhalable particles. The result of this research will assist the museum to come up with a procedure to reduce the effect of airborne particulate matter on museum artefacts. Hence, reducing government expenses through cleaning and maintenance procedure toward effected artefacts. Additionally, this study is beneficial to conservationists, museum management and curators by enlightening them on ways to reduce the damaging effect caused by soiling defects by airborne particulate matter.