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One of the obstacles in utilization of rubber seed oil as a raw material for biodiesel production is contain of high free fatty acid about 12.19%. The process of biodiesel production from rubber seed oil would be more effective and efficient if done through a process of esterification and transesterification using heterogeneous acid catalyst because it is less corrosive, easily separated, and can reduce environmental pollution. However, the catalyst is relatively expensive. Therefore, it needs more economic alternatives. One of which is a heterogeneous acid catalyst from rice water. There are three production process, extracting the oil from seeds, catalyst preparation, and biodiesel production. The results of Biodiesel product contains methyl ester compounds of palmitic acid and oleic acid after going through transesterification process which means that the reaction was run. The EDS test results reported an SO3 group as part of a cluster -SO3H of catalyst from rice water.