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Lots of duku (Lansium domesticum Corr.) Rupit trees have died in the past ten years. However, no high yielding seedling is available for replanting. In this respect, in vitro propagation offers a perfect solution because it allows growers to get millions of pathogen-free and uniform seedlings at a very short of time for rootstocks. The objective of thi experiment was to have the best combination of 2,4-D and BAP in inducing the growth of duku explants. The experiment was conducted from February to June 2018 at the Tissue Culture zlaboratory of the Department of Agronomy and laid in Completely Randomized Design. Seeds of duku were grown in MS media supplemented with 2,4-D (1, 2, 3 ppm) and BAP (2,3,4 ppm). The results showed that all of the media tested produced callous, with different time of formation and qualities. Also, all media produced shoots, but only MS media supplemented with 2 ppm 2,4-D and 2 ppm BAP produced leaves. However, no media produced any root.