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Preservation of natural resources in Subak Guama, must pay attention to ecological balance. Subak plays an important role in the development of agriculture in Bali. One of them is the management of water resources and soil fertility not only the responsibility of the government but also the responsibility of the local community The purpose of this study is to conserve natural resources through ecological balance based on the fertility of land and water resources. The research location was in Subak Guama, Tabanan, Bali Province. The research method used is an exploratory survey method by conducting an ecological survey based on the level of land hunting and water quality that has been carried out by Guama Subak members. The survey was conducted using a questionnaire for implementing the Ecology component. Data were analyzed descriptively. Each ecological component is described to obtain components that have a significant effect on the preservation of natural resources. The results showed that the preservation of soil resources had a significant effect on cropping patterns, pesticides, and fertilizer composition. Preservation of water resources has a significant effect on the management of irrigation networks and water harvesting.