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Anaerobic bacteria in the gingival sulcus is critical for initiation and development of periodontal disease. In addition to periodontal regular procedures, supporting procedure such as irrigation with chlorhexidine or hydrogen peroxide were commonly performed by dental practitioners. Pharmaceutical companies provide several modified irrigating solutions for better efficacy and convenience. However, there are still insufficient evidence for each modification. The purpose of this study is to evaluate the effectiveness in utilizing combined solution of chlorhexidine and hydrogen peroxide toward bacterial growth inhibitory capacity. This research was conducted as an experimental laboratory. The isolated bacteria from human gingival sulcus was taken as a targeted-bacteria. The experiment was performed by using disc-diffusion method on blood agar plate which followed by evaluating the bacterial inhibitory zone. Solutions for the treatment were 0.2% chlorhexidine and 0.2% chlorhexidine combined with 3% hydrogen peroxide. Homogeneity test showed that the data were normally distributed, and the variant analysis showed that the F value was higher than Ft with p<0,05. These results suggested that there was a significant bacterial growth inhibitory capacity among groups. Post-hoc analysis showed that 0.2% chlorhexidine solution gave the higher mean-difference. Therefore, it indicated higher effectiveness in inhibiting anaerobic gingival sulcus bacteria growth capacity.