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A Bedside monitor is an equipment used to monitor patients condition through some parameters that needs sustainable monitoring so that the patients condition is still monitored. Generally, there are several parameters in a Bedside monitor, i.e. Heart signal, Heart rate, respiration, body temperature, non invasiv blood pressure (NIBP), and SPO2. Heart signals can be used to detect patients heart condition such as: whether there is a heart attack or not, whether the heart is defected or not, whether the heart beat is normal or not, and whether the heart has received blood with sufficient oxigen. To help medical staffs and peramedics in hospitals in monitoring patients heart, previous research has been done entitled Design of Cardiograph Machine Based on Atmega Microcontrol (Sience & Irianto, 2016). However, it still has weaknesses. One of them is that the equipment can only monitor point to point heart signals, meaning that one ECG is only used to monitor one patient (not applying multipoint to point). Further, it is completed by creating Design of heart signal based on telemetry (Irianto, 2017). In the research, there are several ECG machines that function as monitor heart signal only. This research is development of previous research that is Design of bedside monitor based on microcontoller can be monitor 5 parameter such as Heart signal, Heart rate,Temperature, Respiration and SPO2. The design of this research applies quasi experimental design. The free variable is ECG phantom or human, and the dependent variable is bedside monitor. The research instruments are calibration equipments of ECG signal, temperature, and respiration. The result of heart signal lead 2 is not differened with the standard and the result of heart rate lead has uncertainty (Ua) 0 for Lead 2; which is still under the tolerance number (0,5). The result of temperature measurement of 5 samples with 5 measurements is 3 samples has deviation standard and uncertainty 0 (zero), whereas 2 samples has uncertainty 0,76 (higher than 0,5). This condition is influenced by patients movements, so the sensor attached on the patients body does not fit with the standard installation. The result of respiration measurement has accuracy 98 percentage, whereas the result of SPO2 after compared with standard calculation has deviation standard and uncertainty under 5 percentage that is STD 0,894427; 0,547723; 0,44; Ua 0,4; 0,244949; 0,2 and 0,2. Ovrall, it can be concluded that Desaign of bedside monitor based on microcontrol is feasible and the mesurement result of heart signal Lead 2, heart rate, temperature, respiration, SPO2 can be presented on PC.