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This study aimed to determine the effect of manipulated cicada sound frequency on evapotranspiration and growth rate of cayenne pepper (Capsicum annum L.). The manipulated cicada sound was exposed with a frequency of 3000Hz, 4000Hz, 5000Hz and untreated under a completely randomized design. The exposure is performed every day in 60 minutes in the coldframe chamber for 43 days. Evapotranspiration, plant height and number of leaves are parameters that can be used as indicators of plant growth. The calculation of reference evapotranspiration was done using the Penman Mointeith method. From this study, it was found that the reference evapotranspiration on in the research site had met the standard of cayenne pepper cultivation. The results showed that the actual optimal evapotranspiration of cayenne pepper occurred in plants that were exposed to a frequency of 5000Hz which was equal to a 4,95 mm/day. While the most optimal growth in plant height and number of leaves occurred in plants that were exposed to a frequency of 5000Hz with an average height growth of 3,02 cm and the number of leaves increasing by 1-2 strands within 3 days. Statistically, the exposure of manipulated cicada sound has a significant influence on the actual evapotranspiration and the growth of cayenne pepper.