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Microplastics (MPs) is the most contamination problem on the marine environment. Small pelagic fish like anchovies are one of the organism which exposured by microplastics, yet reported contamination on Indonesian anchovies is lacking. Here, the investigation result from anchovies digestive tract (Stolephorus spp.) sampled (n = 15). The total microplastics contamination from Talisayan harbor, East Kalimantan is 366 ± 3,51 particles/individual. Kind of microplastics size range detected as categories: < 20 µm, 20-50 µm, 50-500 µm, 500-1000 µm, and >1000 µm. Most of microplastics shapes from Talisayan harbour are microfilm (50%) and microfiber (29,59%). The type of polymers are confirmed by Fourier transform infrared (FTIR) spectroscopy as polypropylene, high-density polyethylene and foamed polystyrene nylon. Our findings reported that spesific size range (50-500 µm) is the most majority of microplastics size range which are ingested by anchovies from Talisayan harbor. The microplastics are more dangerous if another contamination found together on it. On this investigation, the mercury contamination also tested and the result showed as not detected mercury contaminant. The anchovies are had the economic value and also small pelagic fish which are play as prey for the other big fish. The exposured are possible to the human by contaminated seafood diet.